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Stem Cell Procedures for Getting Back on the Field Faster

Sports injuries are a common problem faced by athletes. Some of these injuries can even prove to be career ending as well. However, the latest developments in medical science have offered athletes a chance to get back on the field faster.

One of these developments is stem cell therapy for sports injuries. This therapy makes use of stem cells for treating the common injuries that are faced by an ath

Platelet Rich Plasma Clinic

Hines Ward recovered in only 2 weeks from a knee MCL sprain with the help of PRP and helped the Steelers win a Super Bowl!

lete during his career. Stem cells have been found to be very effective in treating soft tissue injuries that frequently happen on the sports field and can help athletes in recovering quickly from them as well.

Stem Cells for Ligament Injuries

Stem cells have the remarkable capability of turning into any kind of cell that is required by the body. This quality of theirs makes them ideal for treating injuries that damage the tissues or ligaments of the athletes.

Stem cells can turn into the cell of the particular tissue or ligament that has been damaged in the injury and can thus help in its regeneration. This way the stem cells for ligament injuries can speed up the recovery process of an athlete who had injured his ligament while he was on the field and help him in returning to the field quickly.

Options for stem cell procedures for ligament injury include bone marrow or fat derived stem cell procedures, or amniotic derived stem cell injections.

Stem Cells for Tendonitis

Tendonitis is another sports injury that damages the soft tissues of the athletes, mainly their tendons which are attached to the muscles. Tendonitis usually takes a lot of time to heal and the recovery process can take months or even years. This is the reason why tendonitis is considered a career threatening injury. However, stem cells for tendonitis can help an athlete in getting back on the field in a relatively short time period. In this treatment method, the athlete’s affected tendons are injected with stem cells. These stem cells differentiate into cells, which repair the tendon damage and help the athlete in making a quick recovery from his injury.

One of the most interesting facets of stem cell therapy is the ability of the biology to help heal the tendon back to its pre-injury condition. This potential is very different from the body’s natural healing capability. Left to “its own devices”, the healing is not 100%, which can lead to future injury. The stem cells can heal the injuries faster, quicker and get the tissue back to 100%.

Platelet Rich Plasma TherapyStem Cells for Stress Fractures

Stress fractures are part and parcel of an athlete’s life. Since he has to constantly perform physically demanding activities, the elevated levels of stress can result in a fracture. These fractures need time to heal and an athlete might face a lot of time on the sidelines if he gets a stress fracture.

The discovery of stem cells for stress fractures is a hopeful sign for athletes as this treatment can help them in recovering from the fracture within weeks. The stem cells can turn into bone cells and repair the fractured bone within a short period of time. Thus, if you are an athlete suffering from a stress fracture and wants to get back on the field quickly, then stem cells for stress fractures is your best bet. it can save time and for professional athletes – TIME IS MONEY!

Even though stem cell therapy for sports injuries is relatively new, it has helped many athletes in recovering from their injuries in a short span of time. Research on stem cells is still ongoing and it is being projected that in a few years’ time stem cell therapy would overtake the traditional treatment methods used for sports injuries.

R3 Stem Cell works with medical practices nationwide to provide regenerative medicine therapies. This includes sports injuries, degenerative arthritis, diabetic wounds, rotator cuff injuries and more. To find a location close to you, call (855) GET-STEM today!

PRP Therapy for Shoulder Injuries

Platelet Rich Plasma TherapyPlatelet-rich plasma therapy, also known as PRP, is becoming a commonly used method of treatment for those with shoulder injuries and other types of similar injuries to the musculosketeletal system. This type of treatment is often used due to its ability to repair ligaments, tendons, and tissues within the body that have become damaged due to different health conditions or accidents.

PRP offers both pain relief and the ability for the area to heal much quicker than it would naturally. Due to the effectiveness of PRP, it is replacing the need for surgical treatment in many patients, which prevents long recovery periods that can last up to six months or longer. Also, PRP allows the person who gets the treatment to continue on with his or her regular lifestyle routine.

What is PRP Therapy?

PRP therapy is provided in the form of an injection, and the injection is created from a sample of your own blood. There are many different components within your blood, and by separating the blood with the use of a fasting, rotating device, the platelets can be removed from the blood and used for the injection. Each injection used for the PRP therapy process is created solely for the patient who is receiving it. Additionally, the injection is generally given in an outpatient facility, due to the ability for the PRP Therapy for back painprocess to be completed within a few short hours.

How the PRP Injection is Made

Depending on the area where it is being used, the PRP injection will be provided with the use of a syringe. When it is being used as a form of therapy for shoulder injuries, the injection is made into the area of the shoulder that is damaged, and also to areas around the damaged area. This helps to increase healing activity. Additionally, the area may be numbed with a local anesthetic and is generally painless.

Prior to the PRP injection, the doctor will give you information on how to prepare for the injection. After the injection is made, the patient will be monitored to ensure there are no adverse effects. However, the chance of an issue occurring due to the injection is low because the body is able to use the blood easily when given from the patient who is receiving it.

The Benefits of getting PRP Therapy

While certain surgeries can be used as a method to try and correct damage that occurs due to a shoulder injury, this method of treatment can sometimes cause the issue to get worse, which can lead to difficulty with using the arm. The shoulders play an important role in proper usage of the arm area, and by allowing the shoulder to heal with PRP therapy, as it would naturally, the chance of success if much higher. Healing is beneficial for those of any age, but it is especially ideal for those of a young age who need to take part in a lot of activity on a daily basis, which can be painful when there is damage present within the shoulder area.

How Often Treatment Occurs

PRP treatment is effective within the first 48 hours in most cases, and pain relief can last up to one year. However, the amount of time it takes for the area to heal from the injury will vary based on each unique case. Within the time that treatment is provided, the treatment is generally given every six months, and then, any process of regression within the shoulder area is monitored to ensure the treatment method is working correctly.

R3 Stem Cell works with medical practices nationwide, providing regenerative medicine therapy for patient benefit. The stem cell and PRP therapy has been working exceptionally well for degenerative arthritis in the shoulder, knee, hip, ankle along with tendonitis and ligament injuries.

To find a participating R3 Stem Cell center closest to you, call (855) GET-STEM today!

FAQs on Regenerative Medicine

What is regenerative medicine?

Regenerative medicine refers to the field of medicine that focuses on the replacement or regeneration of human cells, tissues and organs in order to establish normal function. Current modalities such as steroid injections only mask the injury by suppressing inflammatory and injury response, while regenerative Stem Cell Therapymedicine offers a more definitive resolution of the injury.

The field focuses on developing interventions that promote regeneration of damaged tissues and organs, either through replacement of damaged tissue or through the stimulation of the body’s growth and repair mechanisms to heal damaged tissues and organs.

Another innovative approach being explored by regenerative medicine is the engineering of tissues in the laboratory to develop functional organs for implantation. This approach is considered as the possible long-term solution for the chronic problem of insufficient organs available for donation and transplantation. Furthermore, if the field is able to successfully develop tissue engineering and transplant generation from patients’ own cells, there will be no more concerns about transplant rejection or graft-versus-host disease.

Are stem cells used in regenerative medicine?

Stem cells are but one of the many therapeutic modalities that are being offered by regenerative medicine. Direct injection of stem cells or progenitor cells as stem cell therapy is done in this field, although approaches such as inducing regeneration through bioactive molecules or infused cells have also been developed. Stem cells have also been used in tissue engineering for organ transplantation.

What are other therapies offered by regenerative medicine?

Chronic Wound Healing

Stem Cell therapy for diabetic woundsOther therapeutic interventions developed in the field of regenerative medicine include the use of heparin sulfate analogues for wound healing or biologic meshes for inguinal hernia repair. Heparan sulfate analogues are used to heal chronic wounds by replacing the degraded heparan sulfate found at the site of injury.

Healing of the damaged tissue is stimulated through the production of growth factors and cytokines in the extracellular matrix. Biologic meshes have been used with success in surgical repair, and are associated with better tolerance than synthetic material.

Another regenerative medicine treatment offered for difficult to heal wounds is amniotic stem cell therapy. This treatment has shown excellent results for helping heal diabetic ulcers and wounds that just will not heal.

Cord blood injection

The use of autologous (i.e., the patient’s own) cord blood stem cells has found interesting uses. These cells have the advantage of being safe from any immune response (as they would be recognized as “self” cells), and they have unique characteristics compared to other varieties of stem cells.

Type I Diabetes

Type I diabetes results from an autoimmune response to the cells in the pancreas that produce insulin. There is ongoing research to assess the validity and effectiveness of slowing the loss of insulin production with infusion of cord blood stem cells.

Cardiovascular repair

The stem cells harvested from cord blood show great promise in cardiovascular repair. Current studies in animals have shown that cord blood stem cells can selectively migrate to dysfunctional cardiac tissue, promoting healing and improving vascular function and blood flow at the site of the injury. Overall heart function has also been demonstrated to improve with this therapy.

Central nervous system repair

Research has shown that brain injury can be treated with cord blood stem cells. These cells have been shown in animal models to migrate to the area of brain injury following intravenous injection, promoting healing and improving neural function. Human cord blood stem cells administered to animals suffering from stroke has been shown to stimulate the growth of new blood vessels, as well as differentiation of neurons, in the brain. The effectiveness of this therapy will have profound implications in the treatment of strokes.

Cerebral palsy and other forms of brain injury can also be treated with autologous cord blood infusions. There is still an ongoing study to assess the validity of this intervention, but early feedback is encouraging.

Platelet Rich Plasma Injections

Platelet rich plasma (PRP) therapy has been found to have great use in promoting healing of injuries, particularly those to the joints, ligaments, tendons, muscles, bones and other tissues. It involves the PRP Therapypreparation of PRP from the patient’s own blood, and injection to the site of injury. Positive outcomes have been consistently reported, and the therapy finds wide application across a broad spectrum of musculoskeletal injuries and conditions.

PRP therapy has been shown to increase the number and viability of cells that repair and reshape cartilage and bone, as well as to promote the reproduction of growth factors and repair proteins. PRP is thought to modulate the gene expression of cells such as chondrocytes, synoviocytes, macrophages, and mesenchymal stem cells, and thereby activate a state of growth and repair. For tissues that enter the anabolic state, this means less inflammation and pain.

Bone Marrow and Fat Derived Injections

These injection according to involve material obtained from one’s own body, processed immediately, and then injected into a person’s problem area. These are low risk, outpatient procedures that have been showing great promise in small studies.

R3 Stem Cell works with clinics nationwide on stem cell therapy for musculoskeletal conditions. These treatments  are all outpatient and may help with pain relief from arthritis, disc disease, sports injuries, tendinitis, ligament injury and more.

Simply click on the Find a Doctor tab to find a clinic close to you or call (844) GET STEM today!


Christ GJ, Saul JM, Furth ME, Andersson KE. The pharmacology of regenerative medicine. Pharmacol Rev. 2013 Jul 1;65(3):1091-133. doi: 10.1124/pr.112.007393.

Orlando G. Regenerative medicine technology applied to gastroenterology: current status and future perspectives. World J Gastroenterol. 2012 Dec 21;18(47):6874-5. doi: 10.3748/wjg.v18.i47.6874.

Packer C, Boddice B, Simpson S. Regenerative medicine techniques in cardiovascular disease: where is the horizon? Regen Med. 2013 May;8(3):351-60. doi: 10.2217/rme.13.21.

Terzic A, Nelson TJ. Regenerative medicine primer. Mayo Clin Proc. 2013 Jul;88(7):766-75. doi: 10.1016/j.mayocp.2013.04.017.

FAQs on Amniotic Stem Cell Therapy

What are amniotic stem cells?

Amniotic stem cells are extracted from the amniotic fluid via a process called amniocentesis or after a c-section from a consenting donor. This procedure allows the harvest of stem cells without harming the embryo, although there is a small risk of pregnancy loss during or following the procedure. The sparing of the embryo makes the harvest of amniotic stem cells more acceptable than embryonic stem cells.

Stem Cell InjectionsBy harvesting the amniotic fluid after the c-section, there is no risk of pregnancy loss.

Amniotic stem cells are pluripotent stem cells that are derived from the mesenchyme. Being pluripotential, these stem cells can differentiate into a wide variety of cell types, including nerves, bone, cardiac muscle fibers, skin and cartilage.

There has been an increase in funding for research on the use of amniotic stem cells for organ regeneration. It is now possible to store stem cells in private stem cell banks, and there has been significant growth in the stem cell industry.

How are stem cells used for treatment?

Stem cell therapy has been approved for very few conditions. Some studies have found that stem cell therapy is effective for immunological and hematological conditions.

There is research that shows amniotic stem cells have promising use in regenerative medicine. As these cells can grow into muscle, cartilage and other forms of tissue, therapies to inject stem cells to stimulate growth and healing are currently being assessed. Cell transplantation technology is currently being developed, and amniotic stem cells appear to be at the forefront of this field.  Stem cells have also been marketed as anti-aging therapy, although research on this particular area has been limited.

Stem cells have been shown to provide promising outcomes in their therapeutic use for progressive and degenerative conditions such as arthritis, tendonitis, and poor-healing wounds. Movement disorders have also been shown to respond to stem cell therapy. It is theorized that these effects are secondary to the increased levels of cell growth and healing factors that accompany stem cell therapy. There are also attempts to use amniotic stem cells for tissue engineering, and for the development of organ transplants.

What are the outcomes for amniotic stem cell injections?

The effectiveness of stem cells as a therapeutic option varies, and more research still needs to be conducted before definitive endorsement of amniotic stem cell therapy can be made. However, this intervention has been shown to provide good outcomes in smaller studies and case reports.Stem Cells for Knee Arthritis

Stem cell injection has been shown to be a well-tolerated procedure that is generally safe. The injection of amniotic stem cells is a minor outpatient procedure that allows a patient to be discharged within the same day.

It should be noted that adverse outcomes are fairly rare with stem cell injection. It also is important to differentiate the actual clinical improvements noted by your physician from any possible placebo effect(s).

Amniotic stem cell injections for musculoskeletal conditions has been increasing in popularity for a number of reasons. The most common reason is because the material is showing excellent results in small studies for arthritis, tendonitis and ligament injury.

Also, amniotic stem cell materials also contain a significant amount of hyaluronic acid along with anti-inflammatory factors. It’s part of what makes amniotic fluid so exciting is the multitude of ingredients!

If you are suffering from pain in the shoulder, hips, knees or ankles, it may be due to arthritis, tendonitis or a ligament injury. Amniotic stem cell therapy may help you with pain relief to avoid surgery and get you back to activities you want!

Click the Find a Doctor tab to find an R3 doctor close to you today!


Abdulrazzak H, De Coppi P, Guillot PV. Therapeutic potential of amniotic fluid stem cells. Curr Stem Cell Res Ther. 2013 Mar;8(2):117-24.

Ge X, Wang IN, Toma I, et al. Human amniotic mesenchymal stem cell-derived induced pluripotent stem cells may generate a universal source of cardiac cells. Stem Cells Dev. 2012 Oct 10;21(15):2798-808. doi: 10.1089/scd.2011.0435.

Petsche Connell J, Camci-Unal G, Khademhosseini A, Jacot JG. Amniotic fluid-derived stem cells for cardiovascular tissue engineering applications. Tissue Eng Part B Rev. 2013 Aug;19(4):368-79. doi: 10.1089/ten.TEB.2012.0561.

Rodrigues MT, Lee SJ, Gomes ME, Reis RL, Atala A, Yoo JJ. Amniotic fluid-derived stem cells as a cell source for bone tissue engineering. Tissue Eng Part A. 2012 Dec;18(23-24):2518-27. doi: 10.1089/ten.tea.2011.0672.

Saito S, Lin YC, Murayama Y, Hashimoto K, Yokoyama KK. Human amnion-derived cells as a reliable source of stem cells. Curr Mol Med. 2012 Dec;12(10):1340-9.

Amniotic Stem Cell Injections for Foot and Ankle Problems

Stem Cells for FootAmniotic stem cells are considered to be undifferentiated or multipotent cells. They have the ability to differentiate into many lineages of cells. These lineages include skin, bone, blood, muscle, and liver cells. The possibility of using amniotic derived stem cells to heal foot and ankle problems is a two-fold solution.

A major health problem faced by patients with diabetes or other diseases causes foot wounds or leg ulcers that can be difficult to cure. With the use of amniotic derived stem cells, patients suffering from these conditions will actually see a cure from something that was previously incurable.

How Do Amniotic Stem Cell Injections Work?

To understand the mechanism of how amniotic stem cell injections work, we must first take a step back and look at how they are harvested and processed then added back into the body. Amniotic stem cells can be harvested through the collection of amniotic fluid during the birth of a child.

Amniotic stem cells are derived from the amniotic fluid and tissues that are found in the womb of a pregnant woman. The fluid is obtained during scheduled c-sections. Normally it would be discarded after the birth.

Once the fluid is collected, the stem cells are filtered out and processed. They can then be added back into the body. With Stem Cells for achillesamniotic derived stem cells, they have the ability to differentiate into many different types of cells.

The stem cells are injected into the afflicted area. There have been many successful cases where tissues have grown back from previously incurable conditions. The treatment of ulcers and wounds to the ankle and foot often do well with an injection of the stem cells, a specialized kind of tissue to be placed over the wound, and debridement before the stem cells are injected.

The amniotic stem cell therapy can help patients avoid surgery and obtain pain relief with achilles tendonitis, ankle arthritis, difficult to heal wounds, plantar fasciitis and more.

The results of this treatment are amazing. In most cases, within two weeks there is a marked improvement in skin condition. There is improvement and complete healing seen in cases where extreme wound conditions were previously seen such as in limb salvage.

Uses of Amniotic Stem Cells

Amniotic stem cells are also used to add in the repair of bone fractures, arthritis, and other conditions affecting the foot and ankle. The treatment is performed in the same manner, with the injection in the afflicted area. For cases where there is a severe break, the injection will occur over a period of time. In cases where arthritis is treated, one injection is given, and then, another follows several weeks later to treat the cause of the inflammation and to give relief from the pain. Stem Cells for plantar fasciitis

The difference in stem cell treatment versus the traditional treatment with cortisone shots is that stem cell therapy treats the root cause of the condition, seeking to change the course of the condition and create healing. Cortisone shots only lessen the pain and do not have the ability to change the course of the actual condition.


Amniotic stem cell injections are still being studied in their effectiveness to treat foot and ankle conditions. Clinical studies have shown marked improvement in patients suffering from wounds caused by conditions such as diabetes. Fractures and other injuries show promise in stem cell treatment as well with therapy occurring on a weekly or bi-monthly basis.

R3 Stem Cell works with foot and ankle specialists nationwide. The treatment works extremely well and is administered as part of numerous research studies. Visit the site today to find a clinic close to you for stem cell therapy for your foot or ankle problem!

Stem Cell Therapy for Plantar Fasciitis

Amniotic Stem Cell Injections for Chronic Back Problems

Amniotic stem cell therapy for chronic back problems is still in the research phase, but it is showing a lot of promise for patients who suffer from arthritis in the back and spinal area. Since the amniotic derived stem cell are not derived from embryonic stem cells or fetal tissue, there are no ethical issues with the treatment.Stem Cells for back pain

The stem cell treatment consists of an injection to the afflicted area. The therapy is designed to actually change the course of the condition and not just mask the pain. This treatment has significant potential for back pain sufferers, and could actually correct structural problems while treating pain and inflammation.

Amniotic stem cells are found in the amniotic fluid of the uterus in pregnant women. These cells are considered pluripotent, because they hold the ability to differentiate into numerous cell lines in various parts of the body. The amniotic fluid is actually inhaled and exhaled by the fetus to properly form the fetal lungs. The cells promote the formation of the body of the fetus and move through the digestive system to form meconium and urine, which ends up expelled in the womb.

The process of acquiring amniotic derived stem cells for treatment is simple. It comes from women who undergo a scheduled c-section, where the amniotic fluid would normally be discarded after the procedure. This fluid can be processed, and the amniotic derived stem cells removed. The material is processed at an FDA regulated laboratory ensuring sterility and regulatory compliance.

Properties of Amniotic Stem Cells

Stem Cells for Disc DegenerationThere is a good reason why amniotic stem cells are used for the treatment of chronic back problems. Amniotic fluid has several properties which enhance the repair and regeneration of tissue. They have anti-inflammatory properties, which aid on pain relief by actually treating the source of the pain instead of treating the symptom like cortisone shots.

These cells contain growth factors which aid in new tissue growth and actually trigger the stem cells to do their proper function. The hyaluronic acid is necessary to lubricate existing cartilage and promote growth of new cartilage. Amniotic fluid has the highest concentration of stem cells in the body, far more than fat or bone marrow. They are also non-allergenic and will not cause any type of rejection reaction to the recipient.

Use of Stem Cell Injections

Amniotic stem cell injections have already been used in several cases such as spinal cord injuries and to promote the formation of an effective scar tissue barrier around spinal injuries. Additionally, they have been used to treat arthritis in the spine, back injuries that previously could only be treated with major surgery, and have the potential to aid in the recovery from paralysis.

The amniotic stem cells are injected into the afflicted area. They are mostly used in growth factor promotion for patients with chronic back problems and to decrease inflammation. It is a less invasive treatment than back surgery, where a needle is used Stem Cell Therapyinstead of a scalpel. The risk of infection is slim to none and the potential benefits are limitless. The use of amniotic stem cells to treat back problems in still in its infancy and will continue to grow as research advances.

R3 works with clinics nationwide that offer regenerative medicine with amniotic derived therapy. The treatments are research based and are industry subsidized, therefore, cost significantly less than at other centers.

Visit the site today to find a treatment center for stem cell injections for back pain, arthritis, ligament or tendon injury today!

Uses for Amniotic Derived Stem Cell Injections

If you have ever had a baby, you probably received unsolicited mail advertising services for banking amniotic stem cells. Since 2009, when the first stem cell bank opened in Medford, MA, cryopreservation and private banking of amniotic fluid stem cells has slowly grown in popularity. For those who are not familiar with amniotic stem cells, they are stem cells derived from the mesenchyme and extracted from the amniotic fluid.

Amniotic stem cells are considered to be a medical miracle, because they can differentiate into a variety of tissue types including bone, muscle, cartilage, skin, nerves, and cardiac tissue. The potential use of these cells gives hope for organ regeneration and recovery from paralysis.

Amniotic stem cells are obtained during a scheduled c-section from consenting donors. Otherwise the fluid is simply discarded – so why not use it for medical benefit? The fluid is processed at an FDA regulated lab prior to usage in individuals that need it.

Why Use Stem Cells?

Amniotic stem cells are a better choice in technologically advanced medical procedures because they do not harm the fetus or the use of an embryo. There is no risk of procedure-related pregnancy loss since the fluid is obtained after birth and not from an amniocentesis. Studies on amniotic fluid have found it rich in epithelial, neural, endothelial, hematopoietic, and mesenchymal stem cells.

Scientists and researchers have genetically engineered liver cells, neural cells, fat cells, heart cells, bone cells, working tracheas, and artificial heart valves by using amniotic stem cells. This indicates that the use of amniotic stem cells in treating a multitude of medical conditions previously thought to be life threatening or untreatable.

Examples of Amniotic Derived Stem Cell Use

Stem cell therapy is a type of intervention therapy which introduces stem cells into damaged tissues to treat an injury or disease. In the world of pain management and orthopedics, stem cell therapy is being used for numerous conditions such as degenerative arthritis, tendonitis, ligament injury and more.

It also works well for wounds that are having difficulty healing, such as in diabetics.

Future Clinical Uses of Amniotic Stem Cells

When it comes to using amniotic stem cells, the uses continue to grow. What is really needed are larger clinical studies. Small studies have shown excellent results so far for regenerative stem cell treatments for musculoskeletal conditions.

Several clinical trials have emerged where subjects are treated with their own stem cell derived bone marrow, and others involve healthy athletes who are treated for osteoskeletal injuries to the bones, joints, or connective tissues. While these treatments have not entered mainstream medical practice, the potential is there for major breakthroughs.

A plethora of athletes have received stem cell therapy and PRP therapy for sports injuries. And the number continues to grow. R3 offers stem cell clinics nationwide with treatments for all types of musculoskeletal injury. Find one close to you today!

Why is Amniotic Tissue Great for Regenerative Medicine?

Regenerative medicine is a relatively new and rapidly evolving field in the treatment of pain management, chronic disease, and injuries previously treatable with only surgery. Stem cell therapy has gained the attention of the scientific community over the past few decades, first with investigational research using embryonic stem cells and now with research focusing on amniotic tissue and amniotic fluid. The stem cells harvested from amniotic derived media are multipotent and able to differentiate themselves into a variety of tissue types. They are characterized by the ability to renew themselves through cell division and the potential to treat many previously untreatable conditions.

The Amniotic Membrane

The majority of research focuses on amniotic fluid and the stem cells that are harvested from this fluid. We must not forget about the stem cell rich amniotic membrane as well. The human amniotic membrane is made up of two types of stem cells: human amnion mesenchymal stromal cells and human amnion epithelial cells. Both of these types of cells display the same properties. When studied in vitro, they differentiate into mesodermal lineages.

The human amniotic membrane has also been used in plastic surgery for the treatment of wounds and corneal problems and in ophthalmology. In recent studies using human amniotic membranes, the tissue patch was successful in helping heal major leg wounds on a dog and to reduce scar adhesion and fibrosis at the site of surgery.

Interventional Pain Management

Human amniotic membranes are also at the forefront of interventional pain management. Using amniotic tissue to treat pain management is actually treating the root cause and not the symptoms, as most other treatments do. Studies concentrating on pain management therapy are investigating the role of amniotic tissue in the healing of tissue damage and inflammation.

One of the more successful studies involved injections of amniotic epithelial cells into an equine digital flexor tendon defect. The result was the growth of new collagen fibers to aid in the repair of that area. The stem cells acted as an activator to the injured or defective area so that growth factors are released and trigger collagen production, aiding in the healing process. Other studies have concentrated on injections of amniotic epithelial cells into the defective Achilles tendon of a sheep. The result was promotion of structural and mechanical recoveries during the early phase of healing.

Future Applications

The primary challenge right now is establishing an efficient method to generate amniotic cell cultures, eliminating the need for refrigeration and centrifugation of fresh samples. This would allow for a continuous supply of clonal lines from amniocentesis samples and birth harvests. Communication is another issue that amniotic derived tissue can solve. In studies where bone marrow derived stem cells were used to attempt to differentiate into another form of cell type, the tissue had to be guided to attempt this differentiation. With human amniotic cells, the communication between host cells and the grafts are pivotal in creating the healing process for damaged tissues.

One future application is using amniotic stem cells to treat babies with congenital heart defects. Although this research is still in the very early phases, it has the potential to treat thousands of babies who are born each year suffering from congenital heart defects. By treating this type of defect, the child would avoid multiple heart operations and the strong possibility of a transplant before the age of one. Research teams are looking at replacing the damaged cells or generating new tissue by augmenting the damaged heart. Researchers understand that the baby’s heart cells are functioning but the heart muscle developed abnormally for reasons unknown.

Stem Cells Derived from Amniotic Fluid: New Potentials in Regenerative Medicine

Human amniotic cells have been used over the past 50 years as a diagnostic tool for prenatal diagnosis of genetic abnormalities in the fetus. Over the past 5 years, however, the potential for using stem cells derived from amniotic fluid has become a reality, especially for treating chronic diseases and conditions.

Stem Cells for Rotator Cuff Tears

Amniotic fluid derived stem cell therapy works well for rotator cuff tears

Amniotic derived stem cells can be harvested from the amniotic sac of a pregnant female. This occurs during child birth or during a procedure called amniocentesis. Since amniocentesis has slight risks, the most common scenario is that amniotic fluid  is obtained during a caesarean section from a consenting donor.

Otherwise it would be discarded, and thankfully amniotic fluid contains a very high concentration of stem cells.

These cells are prized for their ability to form a variety of differentiated cell lines such as blood, bone, muscle, liver, skin, and more. This is unlike the other types of stem cells (other than embryonic), which are only able to differentiate into certain types of tissues.

The Focus

The focus for using stem cells derived from amniotic fluid comes from the ethical battle fought over using embryonic stem cells. Many activists do not like the idea of using stem cells derived from fetuses. Instead, using stem cells derived from amniotic fluid solves this ethical issue. The mother can bank her amniotic fluid or the umbilical cord (which is also rich with stem cells) for later use.

The amniotic fluid found in the womb promotes the develop of the fetus. It is inhaled and exhaled by the fetus in the womb and directly responsible for the development of the lungs. The amniotic fluid promotes the development of the limbs, and passes through the body of the developing fetus re-entering the womb as urine.

How are Stem Cells Derived from Amniotic Fluid used in Research?

Amniotic Stem Cell TherapyThe amniotic cells used in research are primarily donated by mothers in their first trimester of pregnancy. Amniotic derived stem cells were found to have similar properties to embryonic stem cells after tests were performed.

Researchers found that the cells were grown and reprogrammed into a more primitive state when valproic acid was added to the culture medium. Tests run on these cells showed that amniotic derived stem cells have the capability to develop into any type of cell found in the body with a property called pluripotency.

The potential of using amniotic fluid to cure all kinds of diseases, disorders, and injuries is now a reality. Amniotic stem cells not only offer a rich cell line to assist in research but also seem to offer a customized cell supply for newborns and sometimes their siblings. These cells are the medium between embryonic stem cells and adult stem cell lines, such as bone marrow derived stem cells.

Research and the Future of Stem Cells

In the research arena, stem cells are grown in a culture, and there is potential for reengineering and regenerating cells and tissues. Some research has shown that amniotic derived stem cells differentiate using a scaffold or three-dimensional matrix for support.

These cells have been documented to have the ability to differentiate into all types of cells and tissues with a high renewal capacity and no loss of cellular integrity. Stem Cell therapy

The positives outweigh any thought of a negative aspect to using amniotic derived stem cells for treatment and therapy. It is believed that about 38 percent of the population could be treated as a match from stem cells harvested from about 150 donors. This is amazing statistics when you consider the closest approved therapy right now is bone marrow implantation, as well as the odds of finding a donor and the risk of rejection.

R3 Stem Cell offers amniotic stem cell therapy for numerous musculoskeletal conditions including plantar fasciitis, degenerative arthritis, rotator cuff and achilles tendonitis, ligament injury and more. Amniotic derived stem cell therapy offers nonoperative pain relief with the potential to regenerate damaged tissue!

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