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Why is Amniotic Tissue Great for Regenerative Medicine?

Regenerative medicine is a relatively new and rapidly evolving field in the treatment of pain management, chronic disease, and injuries previously treatable with only surgery. Stem cell therapy has gained the attention of the scientific community over the past few decades, first with investigational research using embryonic stem cells and now with research focusing on amniotic tissue and amniotic fluid. The stem cells harvested from amniotic derived media are multipotent and able to differentiate themselves into a variety of tissue types. They are characterized by the ability to renew themselves through cell division and the potential to treat many previously untreatable conditions.

The Amniotic Membrane

The majority of research focuses on amniotic fluid and the stem cells that are harvested from this fluid. We must not forget about the stem cell rich amniotic membrane as well. The human amniotic membrane is made up of two types of stem cells: human amnion mesenchymal stromal cells and human amnion epithelial cells. Both of these types of cells display the same properties. When studied in vitro, they differentiate into mesodermal lineages.

The human amniotic membrane has also been used in plastic surgery for the treatment of wounds and corneal problems and in ophthalmology. In recent studies using human amniotic membranes, the tissue patch was successful in helping heal major leg wounds on a dog and to reduce scar adhesion and fibrosis at the site of surgery.

Interventional Pain Management

Human amniotic membranes are also at the forefront of interventional pain management. Using amniotic tissue to treat pain management is actually treating the root cause and not the symptoms, as most other treatments do. Studies concentrating on pain management therapy are investigating the role of amniotic tissue in the healing of tissue damage and inflammation.

One of the more successful studies involved injections of amniotic epithelial cells into an equine digital flexor tendon defect. The result was the growth of new collagen fibers to aid in the repair of that area. The stem cells acted as an activator to the injured or defective area so that growth factors are released and trigger collagen production, aiding in the healing process. Other studies have concentrated on injections of amniotic epithelial cells into the defective Achilles tendon of a sheep. The result was promotion of structural and mechanical recoveries during the early phase of healing.

Future Applications

The primary challenge right now is establishing an efficient method to generate amniotic cell cultures, eliminating the need for refrigeration and centrifugation of fresh samples. This would allow for a continuous supply of clonal lines from amniocentesis samples and birth harvests. Communication is another issue that amniotic derived tissue can solve. In studies where bone marrow derived stem cells were used to attempt to differentiate into another form of cell type, the tissue had to be guided to attempt this differentiation. With human amniotic cells, the communication between host cells and the grafts are pivotal in creating the healing process for damaged tissues.

One future application is using amniotic stem cells to treat babies with congenital heart defects. Although this research is still in the very early phases, it has the potential to treat thousands of babies who are born each year suffering from congenital heart defects. By treating this type of defect, the child would avoid multiple heart operations and the strong possibility of a transplant before the age of one. Research teams are looking at replacing the damaged cells or generating new tissue by augmenting the damaged heart. Researchers understand that the baby’s heart cells are functioning but the heart muscle developed abnormally for reasons unknown.

Stem Cells Derived from Amniotic Fluid: New Potentials in Regenerative Medicine

Human amniotic cells have been used over the past 50 years as a diagnostic tool for prenatal diagnosis of genetic abnormalities in the fetus. Over the past 5 years, however, the potential for using stem cells derived from amniotic fluid has become a reality, especially for treating chronic diseases and conditions.

Stem Cells for Rotator Cuff Tears

Amniotic fluid derived stem cell therapy works well for rotator cuff tears

Amniotic derived stem cells can be harvested from the amniotic sac of a pregnant female. This occurs during child birth or during a procedure called amniocentesis. Since amniocentesis has slight risks, the most common scenario is that amniotic fluid  is obtained during a caesarean section from a consenting donor.

Otherwise it would be discarded, and thankfully amniotic fluid contains a very high concentration of stem cells.

These cells are prized for their ability to form a variety of differentiated cell lines such as blood, bone, muscle, liver, skin, and more. This is unlike the other types of stem cells (other than embryonic), which are only able to differentiate into certain types of tissues.

The Focus

The focus for using stem cells derived from amniotic fluid comes from the ethical battle fought over using embryonic stem cells. Many activists do not like the idea of using stem cells derived from fetuses. Instead, using stem cells derived from amniotic fluid solves this ethical issue. The mother can bank her amniotic fluid or the umbilical cord (which is also rich with stem cells) for later use.

The amniotic fluid found in the womb promotes the develop of the fetus. It is inhaled and exhaled by the fetus in the womb and directly responsible for the development of the lungs. The amniotic fluid promotes the development of the limbs, and passes through the body of the developing fetus re-entering the womb as urine.

How are Stem Cells Derived from Amniotic Fluid used in Research?

Amniotic Stem Cell TherapyThe amniotic cells used in research are primarily donated by mothers in their first trimester of pregnancy. Amniotic derived stem cells were found to have similar properties to embryonic stem cells after tests were performed.

Researchers found that the cells were grown and reprogrammed into a more primitive state when valproic acid was added to the culture medium. Tests run on these cells showed that amniotic derived stem cells have the capability to develop into any type of cell found in the body with a property called pluripotency.

The potential of using amniotic fluid to cure all kinds of diseases, disorders, and injuries is now a reality. Amniotic stem cells not only offer a rich cell line to assist in research but also seem to offer a customized cell supply for newborns and sometimes their siblings. These cells are the medium between embryonic stem cells and adult stem cell lines, such as bone marrow derived stem cells.

Research and the Future of Stem Cells

In the research arena, stem cells are grown in a culture, and there is potential for reengineering and regenerating cells and tissues. Some research has shown that amniotic derived stem cells differentiate using a scaffold or three-dimensional matrix for support.

These cells have been documented to have the ability to differentiate into all types of cells and tissues with a high renewal capacity and no loss of cellular integrity. Stem Cell therapy

The positives outweigh any thought of a negative aspect to using amniotic derived stem cells for treatment and therapy. It is believed that about 38 percent of the population could be treated as a match from stem cells harvested from about 150 donors. This is amazing statistics when you consider the closest approved therapy right now is bone marrow implantation, as well as the odds of finding a donor and the risk of rejection.

R3 Stem Cell offers amniotic stem cell therapy for numerous musculoskeletal conditions including plantar fasciitis, degenerative arthritis, rotator cuff and achilles tendonitis, ligament injury and more. Amniotic derived stem cell therapy offers nonoperative pain relief with the potential to regenerate damaged tissue!

Why is Amniotic Fluid so Great for Regenerative Medicine?

Amniotic fluid is the protective liquid that is contained within the amniotic sac of a Stem Cell Clinicpregnant mother. Amniotic fluid originates from the mother’s plasma and passes through the fetal membranes by way of osmotic and hydrostatic forces. It is important that the fetus inhales and exhales the amniotic fluid, so that the lungs develop normally.

The amniotic fluid also creates urine, and the fetus secretes and contributes to the formation of meconium. Additionally, amniotic fluid cushions the fetus from any jarring or blows to the mother’s abdomen and promotes the development of the fetus’ skeletal and muscular development.

Amniocentesis Procedure

When fluid is drawn out of the amniotic sac for evaluation, the doctor can determine the fetus’ genetic health. The amniotic fluid contains amniotic stem cells and fetal cells which can be examined for genetic abnormalities.

The procedure of removing amniotic fluid requires a thin needle to pass through the mother’s abdomen, the uterus, and into the amniotic sac where about 20 milliliters of fluid is withdrawn. The doctor uses imaging to determine the fetus’ position to minimize the danger to the fetus. There is less than a 1% risk of miscarriage with amniocentesis.

Amniotic Fluid and Stem Cells

Amniotic fluid contains a considerable amount of stem cells. These are distinguished as amniotic derived stem cells and are considered to be pluripotent, meaning that they can differentiate themselves into various tissues. These stem cells are non-embryonic and Stem Cells for plantar fasciitisare not derived from the embryo, so there is little resistance for using this type of stem cells in research.

Amniotic derived stem cells have shown in research the ability to differentiate into varying cell types, such as brain, bone, and liver tissues. Other types of stem cells do not have this functionality and are only able to differentiate into specified cell types. These types include bone marrow derived stem cells, which only differentiate into blood cells. The amniotic sac is not the only area that contains stem cells. The amnion, umbilical cord, and placenta have all been shown in studies to also contain amniotic derived stem cells, which can be isolated at birth.

Study Regarding Mesenchymal Cells

Amniotic fluid is considered to be so great because of the fairly plentiful source, easy retrieval, and safety associated with the procedure. In studies performed in-vivo, both pluripotent and multi-potent stem cells have been isolated from amniotic fluid. These cells were studied and proven to have the capability to differentiate into kidney and lung tissues, and this proves a potential promise to treat diseases which afflict these tissues.

There has been one case where amniotic derived stem cells have successfully been used for tissue reengineering. In the study, mesenchymal cells were isolated from the amniotic fluid and used to differentiate cartilage in-vivo while maintaining the scaffold for fifteen weeks separating.

Due to research and highly promising findings, amniotic stem banks have popped up around the world, with the main one in Massachusetts, United States. Other reasons amniotic derived stem cells are becoming so popular in research is storage at minimal cost, and over long periods of time with no adverse effects.  Amniotic stem cells are an excellent source of therapy for many genetic disorders which previously were untreatable.

In studies using animal models, these stem cells have shown promise in therapy, but this concept is still fairly new. More research is required to prove how effective amniotic stem cells could be in regenerative medicine. The possibilities are endless, and include therapy for the hematopoietic system, brain, bone, kidney, lung, heart, and other Stem Cell Treatmentimportant tissue types.

With amniotic stem cell treatments, the fluid actually comes after caesarean sections from women who donate it. Otherwise, the fluid would be discarded. Amniocentisis is not necessary for harvesting.

R3 offers amniotic stem cell therapy with stem cell doctors nationwide. Treatments are offered for numerous musculoskeletal conditions including rotator cuff tendonitis, knee arthritis, achilles tendonitis, plantar fasciitis, and more!

5 Things You Should Know About Amniotic-Derived Stem Cell Injections

In the past few years, we have experienced incredible advancement in the field of regenerative medicine, especially because the use of stem cells has been aggressively explored in order to offer solutions for managing pain and orthopedic conditions. Stem cell therapy has been rapidly embraced by the scientific community, both researchers and physicians, because of its amazing potential to heal damaged tissues, joints and other parts of the body. Stem Cell Clinics

One particular area of extensive exploration is the properties and role of amniotic membrane tissue in 21st century regenerative medicine. This increased interest is justified because there is an incredibly high amount of stem cells present in the amniotic tissues, much higher than the amount of stem cells found in adult bone marrow or adipose tissue (fat).

Ok, but what exactly is this amniotic tissue and what are amniotic stem cells?

The amniotic tissue is basically a membrane found in the center layer of the placenta, which is found in the amniotic cavity. This membrane has only one layer of tissue that contains a high number of specialized cells, who actually help the membrane to offer several functions aimed at healing.

During pregnancy, the fetus is surrounded by the amniotic fluid, which has a protective and a feeding role. Following a written consensus from donors, during a caesarean section, stem cells are collected from this amniotic fluid, processed and injected into patients who suffer from a variety of muscular conditions. These are not embryonic cells and there are no ethical concerns. In addition, this fluid is processed at an FDA regulated lab.

Lately, as technology progresses, a considerable amount of patients that have musculoskeletal problems are considering the use of amniotic derived stem cell injections in order to help their body naturally heal itself. And such a choice is justified when you consider the excellent properties of amniotic fluid: natural antiinflamatory response, pain relief, an abundance of growth factors that promote tissue growth, presence of hyaluronic acid that promotes the growth of new cartilages, and a massive concentration of stem cells which allow the body to accept and utilize the fluid without any immune reactions or rejection.

Bottom line is that amniotic derived stem cell injections have been used in the US for a few years now with very promising results, including physicians being successful in treating elbow tendon injuries such tennis elbow and golfer’s elbow, along with rotator cuff tendon injury, spine problems, joint arthritis, wound healing and even corneal injuries.

What’s the most important aspect to remember: amniotic derived stem cell injections help the body to replace damaged cells and also to create new cells, thus giving the body a chance to naturally heal itself. With the help of these injections you don’t just treat the pain itself, but you also treat the source of the pain.

Summary:

  1. Amniotic stem cells have been found to have the potential to heal damaged tissues, joints and other parts of the body.
  2. More and more parents are allowing stem cells to be harvested from amniotic fluid for research and treatment of injuries and medical conditions.
  3. Amniotic-derived stem cells offer an incredible abundance of healing properties including: natural antiinflamatory response, pain relief, an abundance of growth factors that promote tissue growth, and presence of hyaluronic acid that promotes the growth of new cartilages.
  4. There is minimal chance of rejection or allergic reaction to treatment with amniotic-derived stem cells.
  5. This type of therapy has been successfully used to treat tendon injuries such tennis elbow and golfer’s elbow, rotator cuff tendon injury, spine problems, joint arthritis, and wound healing problems.

How does Amniotic Stem Cell Therapy Work for Rotator Cuff and Achilles Tendonitis?

Amniotic stem cell therapy is one of the most effective forms of treatment for many different conditions present within the tendons and joints. The amniotic tissue is contained within the uterus, and its main specialized duty if to offer regenerative healing properties to the fetus while in the mother’s womb.

There is a high amount of stem cells present in amniotic fluid, which makes it easy for the cells to be obtained and used for treatment. While the uses of amniotic stem cell therapy is still being researched, and additional treatment options are presenting themselves, this type of therapy is growing in popularity for conditions such as rotator cuff tendonitis, Achilles tendonitis, and other similar issues.

Amniotic Stem Cell Therapy

The rotator cuff is a structure within the body that works to support the shoulder, and offers significant stability. The cuff is made from a variety of tendons, which work together to create the support. When one of the tendons becomes damaged due to excessive use, trauma, or another issue with the area, the condition is known as tendonitis of the rotator cuff. Surgical procedures do not always help the condition, and could actually cause it to become worse in some cases.

When amniotic stem cell therapy is used for issues with the rotator cuff, there are a few different ways in which the therapy works. For one, when there is an issue present with the tendon, the area will become inflamed, which can lead to pain, swelling, and joint immobility, and even the possibility of more damage occurring. The stem cells derived from the amniotic tissue have shown to help the area repair by providing it with anti-inflammatory effects, growth factors and stem cells. This will decrease the amount of pain present and also take pressure off of the damaged tendon.

Other than taking away inflammation in the area, the amniotic stem cells work to help the tendon to regenerate. The tendon will experience healing in the form of thickening of the tissue within the tendon and regeneration of the structure where damage has occurred. The hyaluronic acid in the amniotic stem cell material also provide lubrication to the tendon, which will prevent further damage from occurring in the area while healing takes place.

The Achilles tendon is the largest tendon in the body, and it goes from the area of the heel, to the top of the calf. When stem cell therapy is used for this area, the healing action is done in the same manner as for the conditions with the tendon in the rotator cuff. While inflammation is sometimes seen with damage in the shoulder area, inflammation is the most common issue to occur when the Achilles tendon becomes damaged. The stem cells offer relief from inflammation, while also providing the area with the ability to repair itself.

How Amniotic Stem Cell Therapy is Provided

Amniotic stem cell therapy is done through the use of injections, which are made directly into the tendon, and they may also be made to the surrounding area. Once the injection is made, pain relief is often provided right away due to the anti-inflammatory properties, and healing will occur at a fast rate as well. However, the rate can differ between patients due to age, the amount of damage present, and the body’s response to the therapy.

R3 Stem Cell Clinics offer first rate, Board Certified doctors who are highly skilled in stem cell therapy. If you or a loved one is suffering from pain in the shoulder, heel, achilles and other areas, stem cell injections  with amniotic fluid derived treatment may help. Call an R3 stem cell clinic today!

What are the Uses for Amniotic-Derived Stem Cell Treatments?

Amniotic tissue is made up from two different cells, and it has many uses that go far beyond the areas of the body where the tissue is naturally present. The unique properties of the tissue have shown that it offers regenerative properties for the body. Those who are researching this topic are interested in learning why exactly amniotic tissue is able to provide restoration to the body when damage has occurred.

The use of the stem cells present within the tissue has shown to be one of the most effective methods of restoring damage to the body. While stem cells can be difficult to collect from certain areas of the body, they are highly concentrated within this tissue, which makes them easily available. Additionally, amniotic-derived stem cells are able to treat a variety of different conditions.

Pain Management from Amniotic-Derived Stem Cells

When an injury occurs within the body, pain is almost always present. In order to provide real pain relief, and not that seen with prescription medication which only hides the pain, the area must be able to heal. When the amniotic-derived stem cells that are taken from the tissue are injected into the damaged area, healing has shown to occur at a rapid rate, which explains how this type of therapy is effective in promoting pain relief for a variety of different conditions.

While the use of amniotic-derived stem cells is still being studied, the use of these cells from the tissue has shown to be effective in treating conditions such as arthritis, soft tissue injuries, and even pain that occurs due to plastic surgery. When the stem cells are used after plastic surgery, they are also effective in providing a much faster rate of healing in the area where the surgery was done. This can even reduce the amount of scarring that occurs due to a slower healing process.

For the treatment of difficult to heal wounds, amniotic fluid derived treatments may help them heal. Diabetic wounds that may not otherwise heal can receive a boost with the amniotic treatments.

Useful Treatments with Stem Cells

While they are commonly used for regular conditions of arthritis, amniotic-derived stem cells are also showing to be effective in treating degenerative arthritis, which was previously known to be an ongoing process, which only gets worse over time. By healing the area that is getting weaker, the process of damage will be reversed, and it will be much harder for arthritis to continue to expand. The injections have also shown to be an effective treatment option for rotator cuff tendonitis, Achilles tendonitis, and a variety of other similar conditions.

Amniotic-derived stem cell treatment has also shown to be very effective in the use of cellular therapy. Due to the immuno-privileged properties that the cells have, there isn’t the risk of the body rejecting the cells, which can occur with certain types of treatment, such as blood transfusions.

Amniotic Tissue Used for Treatments

This tissue used in amniotic-derived stem cell treatment is taken from amniotic fluid, which if often discarded after birth. Amniotic fluid has an incredible amount of stem cells in it, with the extra bonus that none of them are embryonic so there are no ethical concerns.

R3 offers Board Certified doctors who provide regenerative medicine treatments for several conditions in numerous areas of medicine. Amniotic fluid derived stem cell treatments do not involve harvesting from the patient and are performed as an outpatient.

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