Comparing Fresh versus Frozen Stem Cells

Patients frequently ask our providers whether or not we used frozen stem cells or fresh ones. The term for frozen cells is cryopreserved. The answer is we use both depending on the clinic visited for treatment.

In a recent study published in Cell transplantation journal, the authors looked at the differences between cryopreserved me and mesenchymal stem cells, versus non-cryopreserved cells. 

Several variables were evaluated, the first of which looked at cellular viability. The authors noted a 95% viability with non-cryopreserved cells, along with a 90% viability for those that had been cryopreserved. So yes there was a difference in this variable.

The second variable evaluated included, looking at the shape of the stem cells, which is called morphometry.. Interestingly, there were no differences noted between the shape of the mesenchymal stem cells, whether they were cryopreserved or not. The next variable evaluated included looking at the surface markers of Cryopreserved versus fresh mesenchymal stem cells. Interestingly, there were also no differences seen between the markers present. These markers indicate the presence of quality me and Kyle stem cells and were the same.

The last variable included whether or not the stem cells exhibited the ability for try lineage differentiation. This means the ability to differentiate with osteogenesis, chondrogenesis or adipogenesis. Once again, there were no differences seen between the fresh versus frozen stem cells. 

In conclusion, prior preservation, exhibited minimal impact on biological properties, which only amounted significantly in a viability drop of five percentage points with frozen cells. All of the other variables showed equivalence between them. 

Therefore, patients should have no qualms about receiving cryopreserved stem cells as the quality and effectiveness is no different than that scene with fresh stem cells. Call R3 Stem Cell today to set up your free consultation at +1 (844) GET-STEM or email info@r3stemcell.com

Disclaimer: The FDA considers stem cell treatment experimental.